Antioxidant and Radical Scavenging Properties of β-Carotene on Cisplatin Induced Cardiotoxicity
B. Uday Kiran *
Department of Pharmacology, CMR College of Pharmacy, Kandlakoya, Medchal, R R Dist, Hyderabad, 501 401, India
M. Sushma
Department of Pharmacology, CMR College of Pharmacy, Kandlakoya, Medchal, R R Dist, Hyderabad, 501 401, India
K. V. S. R. G. Prasad
Department of Pharmacology, Institute of Pharmaceutical Technology, SPMVV, Tirupathi, Andhra Pradesh, India
V. Uma Maheshwara Rao
Department of Pharmacology, CMR College of Pharmacy, Kandlakoya, Medchal, R R Dist, Hyderabad, 501 401, India
D. Jhansi Laxmi Bai
Department of Pharmacology, CMR College of Pharmacy, Kandlakoya, Medchal, R R Dist, Hyderabad, 501 401, India
V. Nisheetha
Department of Pharmacology, Sri Sai Jyothi College of Pharmacy, Vattinagula pally, Gandipet, Ranga Reddy Dist, Hyderabad-500 075, India
*Author to whom correspondence should be addressed.
Abstract
Objective: To investigate the protective role of β-Carotene against cisplatin induced cardiotoxicity in male albino rats.
Methods: Various biochemical parameters such as Creatine kinase-MB, Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Triglycerides (TG) and Total cholesterol (TC) are being assessed. Also the levels of the in vivo antioxidants such as Reduced glutathione (GSH), Catalase (CAT), and Malondialdehyde (MDA) in the post mitochondrial supernatant of heart were measured. In addition, the histopathological studies were performed to study the protective activity of β-carotene.
Results: Cisplatin administration has shown the elevated levels of the cardiac markers and diminished the endogenous antioxidant levels when compared with the normal rats. β-carotene treatment showed the inhibitory effect on the free radicals showing decreased levels of the cardiac markers like CK-MB, LDH, AST, ALT and ALP. The β-carotene treated rats showed significant (p<0.001) decrease in lipid peroxidation in both prophylactic and curative groups when compared to the cisplatin group. Also showed a significant (p<0.05, p<0.001) increase in the levels of GSH in prophylactic and curative group respectively when compared with the cisplatin group. Both prophylactic and curative groups have shown a significant (p<0.001) increase in the levels of CAT. Further, the histopathological studies confirm the protective effect of β-carotene.
Conclusion: These findings justify the biological and traditional uses of β-carotene as confirmed by its promising radical scavenging activity against cisplatin induced cardiotoxicity.
Keywords: β-Carotene, cardiotoxicity, lipid peroxidation, reduced glutathione, catalase, post mitochondrial supernatant